Stop Stressing the Math: Easy Steps to Peptide Reconstitution

When you first start with research peptides, the math can feel like learning a new language. Numbers, labels, powders, water… it’s a lot. But once you see it step by step, it’s actually very simple.

Step 1: What’s in the Vial?

Look at the label. It will say something like 15 mg or 30 mg.

• That number is how much peptide powder is inside.

Step 2: What is Reconstitution?

Reconstitution just means mixing the powder with liquid (usually bacteriostatic water).

• Why bacteriostatic water? It has a preservative in it (benzyl alcohol) that keeps things stable longer than plain sterile water.

Step 3: The Simple Formula

Here’s the only math you need to know:

Total mg in the vial ÷ Total mL of water you add = Concentration (mg per mL)

Example:

• Vial = 15 mg

• Add 3 mL bacteriostatic water

• 15 ÷ 3 = 5 mg per mL

👉 Too much math? Don’t stress. Use a free online calculator.

Step 4: Start Simple (1:1 Ratio)

At low doses, it’s easiest to keep things super simple:

• Add the same amount of water (mL) as peptide (mg).

• Example: 15 mg vial + 15 mL water = 1 mg per 1 mL.

• This makes the math effortless and beginner-friendly.

But here’s the catch:

• If your dose is higher, this can mean pulling a lot of liquid for one research session.

• That’s where more concentrated mixtures come in handy — using less water so you end up with more peptide per mL.

Example:

• 30 mg vial + 3 mL water = 10 mg per 1 mL.

• Same amount of peptide, but in a much smaller volume of liquid.

Step 6: Use a Calculator (Highly Recommended)

Instead of memorizing formulas, plug the numbers into a calculator:

• Peptide Calculator (Peptide Sciences)

• Peptide Reconstitution Calculator (RUI)

Type in your vial size (mg) and how much water you add (mL) → it shows you the exact concentration.

Step 7: Tips to Make It Foolproof

• Label your vial with: date, water added, and final ratio (mg/mL).

• Store properly: cool, dark, avoid repeated freeze-thaw cycles.

Final Thoughts

The goal is to keep things simple, accurate, and repeatable.

• At low doses → 1:1 ratio keeps math a breeze.

• At higher doses → a more concentrated mix keeps the liquid volume manageable.

And remember: calculators exist so you don’t have to be a math whiz. With a little practice, peptide math will feel second nature.

At TMRS, we’re here to make research feel empowering, not intimidating.